Anti-Transglutaminase 2 抗体 (ab64771)

製品の概要

  • 製品名Anti-Transglutaminase 2 antibody
    Transglutaminase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Transglutaminase 2
  • アプリケーション適用あり: IP, IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human Transglutaminase 2.

    (Peptide available as ab73168.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Lysates: Placenta Tissue, HeLa Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

ターゲット情報

  • 機能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody 画像

  • Transglutaminase 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Transglutaminase 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64771.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 77kDa; Transglutaminase 2, non specific - as present in control (lane 2); 75kDa: We are unsure as to the identity of this extra band.

  • All lanes : Anti-Transglutaminase 2 antibody (ab64771) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 30 seconds
  • ICC/IF image of ab64771 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64771, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml
  • IHC image of Transglutaminase 2 staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64771, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-Transglutaminase 2 antibody (ab64771) 使用論文

ab64771 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixation BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy Monocytes
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Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"