製品の概要

  • 製品名Anti-Transglutaminase 2 antibody
    Transglutaminase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Transglutaminase 2
  • 特異性This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
  • アプリケーション適用あり: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Human
  • 免疫原

    Full length native protein (purified) (Guinea Pig).

  • ポジティブ・コントロール
    • IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/5000.
ELISA 1/300. 1/300 (See Abreview).
IHC-P 1/50 - 1/500.
IHC-Fr Use at an assay dependent concentration. PubMed: 21359202
ICC/IF 1/1000.

ターゲット情報

  • 機能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody 画像

  • This was submitted as part of a review by Dr O Fromigue

  • ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-Transglutaminase 2 antibody (ab421) 使用論文

This product has been referenced in:
  • Sánchez-Lara AC  et al. Feline Chronic Kidney Disease Is Associated With Upregulation of Transglutaminase 2: A Collagen Cross-Linking Enzyme. Vet Pathol N/A:N/A (2014). Cat . Read more (PubMed: 25047228) »
  • Atkinson JM  et al. Inhibition of Thrombin-Activated Fibrinolysis Inhibitor Increases Survival in Experimental Kidney Fibrosis. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 25411467) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (lung)
Specification lung
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
Username

Miss. OLIVIA FROMIGUE

Verified customer

投稿 Aug 08 2005

Thank you for your enquiry and your patience in awaiting a response. This product is raised against tissue TGM (TGM2). I have updated the data sheet to reflect this and added data base links relating to the correct target. If you have nay further quest...

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This antibody was tested in WB with lysates from guinea pig liver. It has never specifically been tested with human squamous epithelial samples. Ab421 does cross-react with Human and it is recommended for use at a dilution of 1/200 - 1/5000.

Ab421 was raised using guinea pig tissue Tgase (full length native protein (purified)). This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-3). If you have any more questions please contact us again.

This antibody has been tested and characterized on formalin-fixed paraffin-embedded liver tissue. We do not have data on frozen sections, however it is likely that this product will work on acetone or ethanol-fixed tissue section as well.

This antibody recognises cellular/tissue transglutaminase. The immunogen used was purified transglutaminase from guinea pig liver. 'TG1' referred to this being the first transglutaminase antibody to be added to our catalog, not the isoform of the a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"