製品の概要

  • 製品名Anti-Transferrin Receptor antibody [YTA 74.4]
    Transferrin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [YTA 74.4] to Transferrin Receptor
  • 特異性This antibody detects mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Tissue/ cell preparation (Mouse). Mouse Concanavilin A activated spleen cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20457837
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 1 x 106 cells.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • 関連疾患Immunodeficiency 46
  • 配列類似性Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • 翻訳後修飾N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • 細胞内局在Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [YTA 74.4] (ab22391) 使用論文

This product has been referenced in:
  • Mukai Y  et al. Induction of endoplasmic reticulum-endosome fusion for antigen cross-presentation induced by poly (?-glutamic acid) nanoparticles. J Immunol 187:6249-55 (2011). IHC (PFA fixed) ; Mouse . Read more (PubMed: 22095716) »
  • Makhro A  et al. Functional NMDA receptors in rat erythrocytes. Am J Physiol Cell Physiol 298:C1315-25 (2010). ICC/IF ; Rat . Read more (PubMed: 20457837) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab22391.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"