Anti-Transferrin Receptor 抗体 [EPR4012] (ab108985)

製品の概要

  • 製品名Anti-Transferrin Receptor antibody [EPR4012]
    Transferrin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4012] to Transferrin Receptor
  • アプリケーション適用あり: IHC-P, WB, IP, Flow Cytmore details
    適用なし: ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Transferrin Receptor aa 1-100. Synthetic peptide against the region close to the N terminal cytoplasmic domain of Human Transferrin Receptor (UniProt P02786).

  • ポジティブ・コントロール
    • Human placenta, TF-1, HeLa and A549 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC.
  • ターゲット情報

    • 機能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • 関連疾患Immunodeficiency 46
    • 配列類似性Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • 翻訳後修飾N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • 細胞内局在Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    Anti-Transferrin Receptor antibody [EPR4012] 画像

    • ab108985 staining Transferrin Receptor in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000 in TBS/BSA/Azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-Transferrin Receptor antibody [EPR4012] (ab108985) at 1/1000 dilution

      Lane 1 : Human placenta cell lysate
      Lane 2 : TF-1 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution


      Predicted band size : 84 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing Jurkat cells stained with ab108985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108985, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-Transferrin Receptor antibody [EPR4012] (ab108985) 使用論文

    ab108985 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (bone marrow (decalcified), placenta)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 min
    Permeabilization No
    Specification bone marrow (decalcified), placenta
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Nov 30 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Human Tissue sections (Placenta)
    Specification Placenta
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Nov 03 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Aug 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"