Anti-Transferrin Receptor 抗体 [B349 (DF1513)] (ab8598)

Abreviews (2)Q&A (11)Specific References (3)

製品の概要

  • 製品名
    Anti-Transferrin Receptor antibody [B349 (DF1513)]
    Transferrin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages.
  • アプリケーション
    適用あり: WB, Flow Cyt, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Cell line KG1.

  • ポジティブ･コントロール
    • Tonsil.
  • 特記事項


    This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • 関連疾患
    Immunodeficiency 46
  • 配列類似性
    Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • 翻訳後修飾
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • 細胞内局在
    Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    画像

    • Western blot - Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)
      Western blot - Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)This image is courtesy of an anonymous Abreview
      All lanes : Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598) at 1/100 dilution

      Lane 1 : Human U2OS cell line, whole cell lysate
      Lane 2 : Human 293T cell line, whole cell lysate
      Lane 3 : African Green Monkey Cos7 cell line, whole cell lysate
      Lane 4 : Hamster CHO cell line, whole cell lysate
      Lane 5 : Rat PC12 cell line, whole cell lysate
      Lane 6 : Mouse 3T3 cell line, whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-mouse antibody

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 7 minutes

      See Abreview

    参考文献

    This product has been referenced in:
    • Perrot G  et al. LRP-1--CD44, a new cell surface complex regulating tumor cell adhesion. Mol Cell Biol 32:3293-307 (2012). Read more (PubMed: 22711991) »
    • Ortiz-Zapater E  et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). Read more (PubMed: 17059402) »

    See all 3 Publications for this product

    ab8598 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    13 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Transferrin Receptor antibody [B349 (DF1513)]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (lung epithelial cells)
    Specification
    lung epithelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Oct 31 2012

    3T3 lysates were negative in western blotting but so were 293 cells. We need an antibody positive for human transferrin receptor and negative for mouse.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    Would these products
    (ab1086, ab84036, ab9179, ab8598) detect Trf1 and Trf2?

    Thank you for your enquiry.

    We have not tested these products (ab1086, ab84036, ab9179) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivity whatso...

    Read More

    I recently ordered the Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598) from you. I have used it at 1:100, in 1% Milk TBST at 4 oC overnight followed by a secondary anti mouse HRP antibody 1:3000. I got no signal from the antidody, a few wea...

    Read More

    Thank you for your inquiry.


    We have confirmedTFR expression in Hep G2, HEL 92.1.7 and TF-1 whole cell lysates using thefollowing conditions:

    Load 50 ug of total protein
    Blocking with 5% milk TBST for one hour at room tem...

    Read More

    I spoke to you today about the Anti-transferrin receptor antibody (ab1086) that did not work for my cell line (Jurkat). I would like to thank you for offering to send us a replacement.  I would be interested in trying the western blot again with ab8598...

    Read More

    Thanks for following up with me. I would be happy to send you a vial of ab8598. I will just need to locate your initial order for ab1086 to process your request. Do you have the purchase order number or Abcam order reference number associated w...

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    Western blot abreview for Anti-Transferrin Receptor antibody [B349 (DF1513)]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS and 293T cell line)
    Loading amount
    30 µg
    Specification
    U2OS and 293T cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 02 2007

    BATCH NUMBER 128772 ORDER NUMBER ? DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE cell lysate of Caco-2 cells PRIMARY ANTIBODY your anti-TfR antibody, catalog number ab8598, lot 128772, dilution 1:100 = 4 ug/mL. 2...

    Read More

    Further to contact with the source of this antibody I have determined that this antibody does not perform well by western blotting. Given that this antibody was detailed as working by western blotting on our datasheets I would like to firstly apolagise...

    Read More

    BATCH NUMBER 128772 ORDER NUMBER ? DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE cell lysate of Caco-2 cells PRIMARY ANTIBODY your anti-TfR antibody, catalog number ab8598, lot 128772, dilution 1:100 = 4 ug/mL. 2...

    Read More

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. Whilst we recommend that our customers use a tonsil preparation as a p...

    Read More

    Regarding the immunogen, has the epitope been mapped? Is it within the extracellular domain?

    The epitope has not been mapped. The cellular loction is cell membrane.

    I have a customer that would like to see a reference or image of this antibody used in IHC. Thanks

    Thank you for your enquiry. Here are some references: Testa, U., wt al 1993. Crit. Rev. Oncog. 4.241-276, Barclay, A.N., et al. 1997. Leucocyte Antigen FactsBook. Academic Press. London Oudemans, et al. 1986 Cancer 58. 1252. Hentze, M.W. and K...

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    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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