Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Transferrin Receptor.
(Peptide available as ab101219.)
Our Abpromise guarantee covers the use of ab84036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa). | |
ICC/IF | Use a concentration of 5 µg/ml. | |
IHC-FrFl | 1/200. |
ab84036 immunohistochemical staining in the rat brain. Sampled were fixed with formaldehyade and heat-mediated antigen retrieval was performed with citric acid. Tissue sections were blocked in 1% BSA for 10 minutes at 21°C before incubation with ab84036 (1/6000) for 16 hours at 21°C. A biotin conjugated goat anti-rabbit IgG secondary was used at 1/250.
ICC/IF image of ab84036 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84036 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab84036 staining mouse liver sections by IHC-FrFl. The animal was perfused with 4% paraformaldehyde, further post fixed with 4% paraformaldehyde overnight and prepared for free floating sectioning. Staining with ab84036 at a 1/200 dilution in 0.1% TritonX with 0.1x PBS and 10% donkey was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody was used as the secondary antibody.
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