Anti-Transferrin Receptor 抗体 [13E4] (ab38171)


  • 製品名Anti-Transferrin Receptor antibody [13E4]
    Transferrin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [13E4] to Transferrin Receptor
  • 特異性This antibody is specific for Transferrin Receptor and the complex between soluble Transferrin Receptor and Transferrin.
  • アプリケーション適用あり: ELISA, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human soluble transferrin receptor.

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.



Our Abpromise guarantee covers the use of ab38171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 機能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • 関連疾患Immunodeficiency 46
  • 配列類似性Belongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • 翻訳後修飾N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • 細胞内局在Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [13E4] 画像

  • ICC/IF image of ab38171 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38171 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing Jurkat cells stained with ab38171 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab38171, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Transferrin Receptor antibody [13E4] (ab38171) 使用論文

This product has been referenced in:
  • Zhang X  et al. Urethral musculature and innervation in the female rat. Neurourol Urodyn 35:382-9 (2016). Read more (PubMed: 25597596) »
  • Sade H  et al. A human blood-brain barrier transcytosis assay reveals antibody transcytosis influenced by pH-dependent receptor binding. PLoS One 9:e96340 (2014). Read more (PubMed: 24788759) »

See all 3 Publications for this product

Product Wall

Thank you for your reply. We do not have any data on the use of this antibody on chinese hamster; however, I have performed an alignment of the human (immunogen) and chinese hamster sequences and have found that there is only 77% identity. Beca...

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Thank you for your email. Unfortunately we have not determined the epitope for this antibody. We only know that it recognizes both human soluble transferrin receptor and complex between soluble transferrin receptor and transferrin. I am...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Met5A)
Specification Met5A
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

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投稿 Nov 21 2007