製品の概要

  • 製品名Anti-Transferrin antibody
    Transferrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Transferrin
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Dog, Human
  • 免疫原

    Human Transferrin Protein

  • ポジティブ・コントロール
    • Rat apotransferrin protein. IF: HepG2 cell line This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/10000. Predicted molecular weight: 77 kDa.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody 画像

  • Anti-Transferrin antibody (ab82411) at 1/10000 dilution + rat apotransferrin
    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ICC/IF image of ab82411 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Transferrin staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82411, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Transferrin antibody (ab82411) 使用論文

This product has been referenced in:
  • Zelenchuk LV  et al. PHEX mimetic (SPR4-peptide) corrects and improves HYP and wild type mice energy-metabolism. PLoS One 9:e97326 (2014). Mouse . Read more (PubMed: 24839967) »
  • Zhao M  et al. Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat model. Proteome Sci 12:42 (2014). WB ; Rat . Read more (PubMed: 25061428) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse pancreatic acinar cells)
Loading amount 30 µg
Specification Mouse pancreatic acinar cells
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20 %)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Oct 02 2012

Thank you for calling Abcam earlier today.
I did a little more searching and I found that we already have the blocking peptide for ab16051 in stock, it is ab16377:
http://www.abcam.com/beta-catenin-peptide-ab16377.html
As for ab82411, I ...

Read More

Blocking buffer has been used as diluent. This particular antibody was tested at 1:2000 using 5%NFD milk/1xPBS/0.05%Tween20. The WB protocol followed is overnight blocking at 4C and 1 hour incubations at room temp with the primary and then with the s...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"