Anti-Transferrin Affibody® Molecule (ab31910)

製品の概要

  • 製品名Anti-Transferrin Affibody® Molecule
  • 特異性ab31910 recongises transferrin.
  • アプリケーション適用あり: ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    -

  • 特記事項

    ab31910 is a recombinant protein produced in E. coli.

    What are Affibody Molecules?
    Affibody® affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface- binding properties. The current Affibody® libraries contains billions of variants. In function, Affibody® molecules mimic antibodies, nature’s own binders to an infinite number of antigens. Compared to antibodies, the most striking dissimilarity of Affibody® molecules is the small size. Affibody® molecules have a molecular weight of 14 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of Affibody® molecules is similar to that of an antibody. The advantages of Affibody® molecules over antibodies are · their small size · the simple structure of the molecules · its robust physical properties · its ability to fold correctly intracellularly · the fast and cost-efficient production in bacteria · the possibility to produce Affibody® molecules through chemical synthesis · the possibility to couple Affibody® molecules in multimeric constructs.

    This product works very well for purification and depletion of transferin from plasma. This Anti-Tranferin Affibody® Molecule is modified with a unique C-terminal cysteine for directed single-point chemical modification, facilitating coupling to matrices. However, tail-to-tail dimers are spontaneously generated via a disulphide bridge between the C-terminal cysteines. Prior to coupling via the C-terminal the Affibody® Molecule needs to be reduced to expose the reactive cysteine residue. Recommended reducing condition is 20mM DTT at a pH above 7.5 and incubation at room temperature for 2 hours. Remove excess DTT by passage through a desalting column, not by dialysis. ab50345 is a secondary antibody suitable for use in the process of detecting this Affibody® Molecule.

    THIS AFFIBODY® MOLECULE REQUIRES CONJUGATION TO A SUITABLE LABEL BEFORE USE. PLEASE REFER TO THE "PROTOCOLS" LINK BELOW.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーpH: 7.4
    Constituents: 0.079% Ammonium bicarbonate, PBS
  • Concentration information loading...
  • 特記事項(精製)ab31910 is >98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Affibody® molecule 備考What are Affibody Molecules?
    Affibody® affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface- binding properties. The current Affibody® libraries contains billions of variants. In function, Affibody® molecules mimic antibodies, nature’s own binders to an infinite number of antigens. Compared to antibodies, the most striking dissimilarity of Affibody® molecules is the small size. Affibody® molecules have a molecular weight of 14 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of Affibody® molecules is similar to that of an antibody. The advantages of Affibody® molecules over antibodies are · their small size · the simple structure of the molecules · its robust physical properties · its ability to fold correctly intracellularly · the fast and cost-efficient production in bacteria · the possibility to produce Affibody® molecules through chemical synthesis · the possibility to couple Affibody® molecules in multimeric constructs
  • 研究分野
  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Apotransferrin
    • Beta 1 metal binding globulin
    • Beta-1 metal-binding globulin
    • DKFZp781D0156
    • PRO1400
    • PRO1557
    • PRO2086
    • Serotransferrin
    • Serotransferrin precursor
    • Siderophilin
    • TF
    • TFQTL1
    • Transferin
    • Transferrin
    • TRFE_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab31910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

This product is ideal as a capture molecule in ELISA assays.

AP Use at an assay dependent concentration.

Anti-Transferrin Affibody® Molecule 画像

  • Standard transferrin was titrated on Anti-Transferrin Affibody® molecule coated plates with a sensitivity of 30 ng/ml.

    QUANTITATIVE ELISA

    The Anti-Transferrin Affibody® molecule can be used as capture reagent in a sandwich ELISA in combination with a rabbit anti-transferrin antibody as the detection reagent. Titration of transferrin gives a sigmoid curve with a sensitivity of 30 ng transferrin/ml (defined as two times background value) and a measurement interval between 100 and 1000 ng/ml.

  • ANALYSIS OF TRANSFERRIN CONCENTRATION IN DEPLETED PLASMA

    The concentration of transferrin in plasma samples previously depleted from transferrin by passage through an Anti-Transferrin Affibody® molecule SulfoLink® Coupling Gel column, was analyzed using the Anti-Transferrin Affibody® ELISA. Flow through samples from 60, 600, 800 and 1200 ul depleted plasma were analyzed, the concentration of transferrin in the samples and the percentage of achived depletion are presented in table 1.

Anti-Transferrin Affibody® Molecule (ab31910) 使用論文

ab31910 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The product you have asked about (ab31910) is an affibody product. These are mimic antibody molecules and this one will bind both to the human transferrin antigen, and also to protein A. Unfortunately, we do not sell any an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"