製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRAK1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (LETEAADLGN DERSKKPGTP GTPGSHDLET ALRRLSLRRE NYLSERRFFE) of Human TRAK1 (NP_001036111).

  • ポジティブ・コントロール
    • HT1080 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    The specific function of TRAK1 remains unknown. It interacts with O-GlcNAc transferase, RHOT1/Miro-1 and RHOT2/Miro-2. It shows high expression in spinal cord and moderate expression in all other tissues and specific brain regions examined.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • 106 kDa O GlcNAc transferase interacting protein antibody
    • KIAA1042 antibody
    • OGT(O Glc NAc transferase) interacting protein 106 KDa antibody
    • OIP106 antibody
    • Trafficking kinesin binding protein 1 antibody
    • Trafficking protein kinesin binding 1 antibody
    • TRAK 1 antibody
    see all

画像



  • Predicted band size : 106 kDa
    Western blot analysis of HT1080 cell lysate labeling TRAK1 with ab89962 at 1.0µg/ml.


  • Predicted band size : 106 kDa
    Western blot analysis of HepG2 cell lysate labeling TRAK1 with ab89962 at 1.0µg/ml.


  • Predicted band size : 106 kDa
    Western blot analysis of HeLa cell lysate labeling TRAK1 with ab89962 at 1.0µg/ml.


  • Predicted band size : 106 kDa
    Western blot analysis of ACHN cell lysate labeling TRAK1 with ab89962 at 1.0µg/ml.
  • Anti-TRAK1 antibody (ab89962) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 106 kDa
    Gel concentration: 6-18%

プロトコール

参考文献

ab89962 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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