The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06-0.125µg for 105-8 cells.
Staining the cell sample in a final volume of 100µl is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Widespread; most predominant in spleen, lung and prostate.