製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 60 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/1000 - 1/5000.
IP 1/50 - 1/200.

ターゲット情報

  • 機能E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
  • 組織特異性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • ドメインThe coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • 翻訳後修飾Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
    Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
  • 細胞内局在Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase TRAF6 antibody
    • Interleukin 1 signal transducer antibody
    • Interleukin-1 signal transducer antibody
    • MGC 3310 antibody
    • MGC:3310 antibody
    • MGC3310 antibody
    • OTTHUMP00000232772 antibody
    • OTTHUMP00000232773 antibody
    • RING finger protein 85 antibody
    • RNF 85 antibody
    • RNF85 antibody
    • TNF receptor associated factor 6 antibody
    • TNF receptor-associated factor 6 antibody
    • TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody
    • TRAF 6 antibody
    • Traf6 antibody
    • TRAF6_HUMAN antibody
    see all

Anti-TRAF6 antibody 画像

  • All lanes : Anti-TRAF6 antibody (ab94769) at 1/2000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human kidney lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human prostate lysate
    Lane 6 : Human breast lysate
    Lane 7 : Human colon lysate
    Lane 8 : Human thymus lysate
    Lane 9 : Human lymph node lysate
    Lane 10 : Human spleen lysate
    Lane 11 : Human lung lysate
    Lane 12 : Human aorta lysate
    Lane 13 : Human epidermis lysate


    Predicted band size : 60 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  • ab94769, at a 1/2000 dilution, staining mouse TRAF6 in lymph node, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue (hematoxylin-eosin counterstain).
  • ab94769, at a 1/2000 dilution, staining mouse TRAF6 in bone marrow by Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue (hematoxylin-eosin counterstain).
  • ab94769, at a 1/2000 dilution, staining mouse TRAF6 in spleen, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue (hematoxylin-eosin counterstain).
  • ab94769, at a 1/2000 dilution, staining mouse TRAF6 in thymus by Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue (hematoxylin-eosin counterstain).

Anti-TRAF6 antibody (ab94769) 使用論文

ab94769 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Anfrage.

Ich freue mich ihnen mitteilen zu koennen dass wir fuer den gesamten November unsere Aktion 4 fuer 3 laufen haben. Bei der Bestellung von 4 Produkten bekommen Sie das guenstigste Produkt kostenfrei.

...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cause...

Read More

Thank you for your reply. I have checked our available stock and unfortunately we only have the lot you have already tried with ab94769. As an alternative, I would like to recommend ab13853, This antibody is also guaranteed to react with mouse in paraf...

Read More

Thank you for contacting Abcam regarding ab94769. I am sorry that you have been experiencing difficulties using this antibody in IHC on paraffin embedded sections. I have reviewed the protocol information you provided and I agree that this product is n...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"