製品の概要
- 製品名Anti-TRAF3 antibody
TRAF3 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to TRAF3
- アプリケーション適用あり: WB, ICC/IFmore details
- 種交差性交差種: Mouse, Human
- 免疫原
A 16 amino acid peptide from near the carboxy terminus of human TRAF3.
- ポジティブ･コントロール
- 3T3 cell lysate.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C.
- バッファーPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - 精製度Immunogen affinity purified
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab62552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. |
ターゲット情報
- 機能Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
- 配列類似性Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. - ドメインThe MATH/TRAF domain binds to receptor cytoplasmic domains.
The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing. - 翻訳後修飾Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.
- 細胞内局在Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
- Information by UniProt
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参照データベース
- Entrez Gene: 7187 Human
- Entrez Gene: 22031 Mouse
- Omim: 601896 Human
- SwissProt: Q13114 Human
- SwissProt: Q60803 Mouse
- Unigene: 510528 Human
- Unigene: 27431 Mouse
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別名
- CAP 1 antibody
- CAP-1 antibody
- CAP1 antibody
see all
Anti-TRAF3 antibody 画像
-
Western blot - TRAF3 antibody (ab62552)Lane 1 : Anti-TRAF3 antibody (ab62552) at 1 µg/ml
Lane 2 : Anti-TRAF3 antibody (ab62552) at 2 µg/ml
Lane 1 : 3T3 cell lysate
Lane 2 : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 65 kDa
Observed band size : 65 kDa -
Immunocytochemistry/ Immunofluorescence-Anti-TRAF3 antibody(ab62552)Immunofluorescence of TRAF3 in 3T3 cells using ab62552 at 2 ug/ml.
Anti-TRAF3 antibody (ab62552) 使用論文
ab62552 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"