製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRAF3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human TRAF3 aa 250-350 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P36941

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HEK293 whole cell lysate as well as the following Human tissue lysates: Fetal Kidney; Fetal Liver; Fetal Lung; Thymus; Spleen; Lymph node; Bone marrow.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能
    Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
  • 配列類似性
    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • ドメイン
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing.
  • 翻訳後修飾
    Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.
  • 細胞内局在
    Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAP 1 antibody
    • CAP-1 antibody
    • CAP1 antibody
    • CD40 associated protein 1 antibody
    • CD40 binding protein antibody
    • CD40 bp antibody
    • CD40 receptor associated factor 1 antibody
    • CD40 receptor-associated factor 1 antibody
    • CD40-binding protein antibody
    • CD40BP antibody
    • CRAF 1 antibody
    • CRAF1 antibody
    • IIAE5 antibody
    • LAP 1 antibody
    • LAP1 antibody
    • LMP1 associated protein antibody
    • LMP1-associated protein 1 antibody
    • MIPT3 antibody
    • TNF receptor associated factor 3 antibody
    • TNF receptor-associated factor 3 antibody
    • TRAF 3 antibody
    • Traf3 antibody
    • TRAF3_HUMAN antibody
    see all

画像

  • All lanes : Anti-TRAF3 antibody (ab110781) at 1 µg/ml

    Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Fetal Liver (Human) Normal Tissue Lysate
    Lane 3 : Lung (Human) Whole Cell Lysate - fetal normal tissue
    Lane 4 : Thymus (Human) Tissue Lysate - adult normal tissue
    Lane 5 : Spleen (Human) Tissue Lysate - adult normal tissue
    Lane 6 : Lymph node (Human) Tissue Lysate - adult normal tissue
    Lane 7 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 8 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa


    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab1107881 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

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参考文献

ab110781 has not yet been referenced specifically in any publications.

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