製品の概要

  • 製品名Anti-TRA2B antibody
    TRA2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRA2B
  • アプリケーション適用あり: WB, IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
    交差が予測される動物種: Rabbit, Horse, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide: MSDSGEQNYG ERESRSASRS GSAHGSGKSA RHTPARSRSK EDSRRSRSKS, corresponding to amino acids 1-50 of Human TRA2B

  • ポジティブ・コントロール
    • Jurkat cell lysate. Human lung tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22437831

ターゲット情報

Anti-TRA2B antibody 画像

  • All lanes : Anti-TRA2B antibody (ab66901) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TRA2B with ab66901 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

Anti-TRA2B antibody (ab66901) 使用論文

This product has been referenced in:
  • Llorian M  et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse . Read more (PubMed: 27317697) »
  • Fu K  et al. Tra2ß Protein Is Required for Tissue-specific Splicing of a Smooth Muscle Myosin Phosphatase Targeting Subunit Alternative Exon. J Biol Chem 287:16575-85 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22437831) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NSC34 cells)
Loading amount 5 µg
Specification NSC34 cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"