製品の概要

  • 製品名Anti-TRA2A antibody
    TRA2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRA2A
  • 特異性This antibody detects endogenous levels of total TRA2A protein.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human TRA2A.

  • ポジティブ・コントロール
    • Hela cells, human brain tissue sections and extracts from HUVEC cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 27, 33, 44 kDa (predicted molecular weight: 33 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
  • 配列類似性Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Phosphorylated in the RS domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AWMS1 antibody
    • HSU53209 antibody
    • htra-2 alpha antibody
    • Putative MAPK activating protein PM24 antibody
    • TRA 2 alpha antibody
    • TRA 2A antibody
    • TRA-2 alpha antibody
    • TRA2 alpha antibody
    • TRA2-alpha antibody
    • TRA2A antibody
    • TRA2A_HUMAN antibody
    • Tra2alpha antibody
    • Transformer 2 alpha antibody
    • Transformer 2 protein homolog antibody
    • Transformer-2 protein homolog A antibody
    • Transformer-2 protein homolog alpha antibody
    see all

Anti-TRA2A antibody 画像

  • All lanes : Anti-TRA2A antibody (ab72625) at 1/250 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Mouse ES (129Sv) whole cell lysate
    Lane 3 : 293T whole cell lysate
    Lane 4 : Mouse ES (129Sv) whole cell lysate
    Lane 5 : 293T whole cell lysate
    Lane 6 : Mouse ES (129Sv) whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 27 kDa (possible non-specific binding),85 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-TRA2A antibody (ab72625) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 27 kDa,44 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1:50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • Immunofluorescence analysis of Hela cells using ab72625 at a 1:500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

Anti-TRA2A antibody (ab72625) 使用論文

ab72625 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse ES cells)
Specification mouse ES cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Nov 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"