製品の概要

  • 製品名
    Anti-TRA-1-81 antibody [TRA-1-81]
  • 製品の詳細
    Mouse monoclonal [TRA-1-81] to TRA-1-81
  • アプリケーション
    適用あり: ICC/IF, WB, IP, ICC, RIA, IHC-Frmore details
  • 種交差性
    交差種: Rabbit, Human
    非交差種: Mouse
  • 免疫原

    Human embryonal carcinoma cell line 2102Ep cl.2A6.

  • ポジティブ・コントロール
    • WB: HUES7 whole cell lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.5
  • 精製度
    Tissue culture supernatant
  • 特記事項(精製)
    Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • ポリ/モノ
    モノクローナル
  • クローン名
    TRA-1-81
  • ミエローマ
    P3x66Ag.8-Sp2/0
  • アイソタイプ
    IgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The Podocalyxin gene encodes a member of the sialomucin protein family, and the encoded protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: playing a role in hematopoetic cell differentiation, binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements and being expressed in vascular endothelium cells and binding to L-selectin.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • Gp200 antibody
    • PC antibody
    • PCLP antibody
    • PCLP1 antibody

画像

  • Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 200-400 kDa


    Exposure time : 20 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg

    Secondary
    Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 200-400 kDa


    Exposure time : 3 minutes
  • ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.

    DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.

    See Abreview

  • ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.

    See Abreview

参考文献

This product has been referenced in:
  • Li Y  et al. Abnormal Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Partially Mimicked Development of TSC2 Neurological Abnormalities. Stem Cell Reports 8:883-893 (2017). IF ; Human . Read more (PubMed: 28344003) »
  • Ding C  et al. Different therapeutic effects of cells derived from human amniotic membrane on premature ovarian aging depend on distinct cellular biological characteristics. Stem Cell Res Ther 8:173 (2017). WB ; Human . Read more (PubMed: 28750654) »

See all 26 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Embryonic stem cell, HUES7)
Specification
Embryonic stem cell, HUES7
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Ms. Fiona Lewis

Verified customer

投稿 Oct 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cells)
Specification
embryonic stem cells
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Maria Keramari

Verified customer

投稿 Dec 06 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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