製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TPX2
  • アプリケーション
    適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Mixture of 3 KLH conjugated synthetic peptides selected from C-terminal region with phenylalanine at position 624, C-terminal region with serine at position 729, and center region of human TPX2.

  • ポジティブ・コントロール
    • Human placenta lysate. Human hepatocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20599806

ターゲット情報

  • 機能
    Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
  • 組織特異性
    Expressed in lung carcinoma cell lines but not in normal lung tissues.
  • 配列類似性
    Belongs to the TPX2 family.
  • 発生段階
    Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C20ORF1 antibody
    • C20orf2 antibody
    • Chromosome 20 Open Reading Frame 1 antibody
    • Differentially expressed in cancerous and non-cancerous lung cells 2 antibody
    • Differentially expressed in cancerous and noncancerous lung cells 2 antibody
    • Differentially expressed in lung cells 2 antibody
    • Differentially expressed in lung cells antibody
    • DIL 2 antibody
    • DIL-2 antibody
    • DIL2 antibody
    • FLS353 antibody
    • GD:C20orf1 antibody
    • HCA 519 antibody
    • HCA519 antibody
    • HCTP 4 antibody
    • HCTP4 antibody
    • Hepatocellular carcinoma associated antigen 519 antibody
    • Hepatocellular carcinoma-associated antigen 519 antibody
    • p100 antibody
    • Preferentially expressed in colorectal cancer antibody
    • Protein fls353 antibody
    • REPP 86 antibody
    • repp86 antibody
    • Restricted expression proliferation associated protein 100 antibody
    • Restricted expression proliferation-associated protein 100 antibody
    • Targeting protein for Xklp2 antibody
    • TPX 2 antibody
    • TPX2 antibody
    • TPX2 microtubule associated homolog antibody
    • TPX2 microtubule associated protein homolog antibody
    • TPX2 microtubule nucleation factor antibody
    • TPX2_HUMAN antibody
    see all

画像

  • Anti-TPX2 antibody (ab71816) at 1/100 dilution + human placenta lysate at 12.5 µg

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 110 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • ab71816 at 1/50 dilution, staining TPX2 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

参考文献

This product has been referenced in:
  • Chen QI  et al. TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance. Oncol Lett 11:3515-3521 (2016). WB, IHC-P . Read more (PubMed: 27123144) »
  • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »

See all 4 Publications for this product

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