製品の概要

  • 製品名Anti-TPR antibody
    TPR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TPR
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 2300-2349 of Human TPR (NP_003283.1).

  • ポジティブ・コントロール
    • Human Breast Carcinoma, Colon Carcinoma, Hodgkins Lymphoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Squamous Cell Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma and mouse Squamous Cell Carcinoma tissue.
  • 特記事項

    Concentration is optimized for IHC and not determined

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
ICC/IF
  • 追加情報ICC/IF: 1/100 - 1/500. Permeabilization with Triton-X 100 is recommended.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
    • 組織特異性Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
    • 関連疾患Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
      Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Nucleoprotein TPR antibody
      • tpr antibody
      • TPR_HUMAN antibody
      • translocated promoter region (to activated MET oncogene) antibody

    Anti-TPR antibody 画像

    • ab84516 at a 1/250 dilution, staining in human TPR in human prostate carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
    • ab84516 at a 1:250 dilution, staining mouse TPR in mouse squamous cell carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
    • ab84516 at a 1/250 dilution, staining human TPR in NBF-fixed asynchronous HeLa cells by immunocytochemistry. Detection: anti-rabbit IgG-FITC conjugate used at a dilution of 1/100.
    • ab84516 at a 1/250 dilution, staining TPR in human breast carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.

    Anti-TPR antibody (ab84516) 使用論文

    This product has been referenced in:
    • Guo Y  et al. Concentration-dependent lamin assembly and its roles in the localization of other nuclear proteins. Mol Biol Cell 25:1287-97 (2014). ICC/IF ; Mouse . Read more (PubMed: 24523288) »
    • Umlauf D  et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J Cell Sci 126:2656-67 (2013). Human . Read more (PubMed: 23591820) »

    See all 2 Publications for this product

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