製品の概要

  • 製品名Anti-TPR antibody
    TPR 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to TPR
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: MAAVLQQVLE RTELNKLPKS VQNKLEKFLA DQQSEIDGLK GRHEKFKVES EQQYFEIEKR LSHSQERLVN ETRECQSLRL ELEKLNNQLK ALTEKNKE, corresponding to amino acids 1-99 of Human TPR

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 267 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 22253824

ターゲット情報

  • 機能Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
  • 組織特異性Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
  • 関連疾患Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
    Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Nucleoprotein TPR antibody
    • tpr antibody
    • TPR_HUMAN antibody
    • translocated promoter region (to activated MET oncogene) antibody

Anti-TPR antibody 画像

  • TPR antibody (ab58344) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebellum.


  • Predicted band size : 267 kDa
  • Immunofluorescence analysis of HEK293T cells, 48 hours after transfection with non-specific siRNA (upper panels) or TPR siRNA (lower panels). TPR was stained using ab58344 (green). Nuclei were stained using DAPI (blue).


  • Predicted band size : 267 kDa

    Image from Rajanala K et al., PLoS One. 2012;7(1):e29921. Epub 2012 Jan 13. Fig 1.; doi:10.1371/journal.pone.0029921; January 13, 2012, PLoS ONE 7(1): e29921.

  • Overlay histogram showing HeLa cells stained with ab58344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58344, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-TPR antibody (ab58344) 使用論文

This product has been referenced in:
  • Rajanala K & Nandicoori VK Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. PLoS One 7:e29921 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22253824) »
  • Tarazón E  et al. Heart failure induces significant changes in nuclear pore complex of human cardiomyocytes. PLoS One 7:e48957 (2012). WB ; Human . Read more (PubMed: 23152829) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Human Cell (U2OS)
Specification U2OS
Permeabilization Yes - NP40
Fixative Formaldehyde
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投稿 Sep 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"