製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to TPP1
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 195-305 of Human TPP1

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab195234 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
  • 組織特異性
    Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
  • 関連疾患
    Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles.
  • 配列類似性
    Belongs to the peptidase S53 family.
  • 翻訳後修飾
    Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity.
  • 細胞内局在
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell growth inhibiting gene 1 protein antibody
    • Cell growth-inhibiting gene 1 protein antibody
    • Ceroid lipofuscinosis neuronal 2 antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile (Jansky Bielschowsky disease) antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile antibody
    • CLN 2 antibody
    • CLN2 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • Growth inhibiting protein 1 antibody
    • LPIC antibody
    • Lysosomal pepstatin insensitive protease antibody
    • Lysosomal pepstatin-insensitive protease antibody
    • MGC21297 antibody
    • TPP 1 antibody
    • TPP I antibody
    • TPP-1 antibody
    • TPP-I antibody
    • Tpp1 antibody
    • TPP1_HUMAN antibody
    • TPPI antibody
    • Tripeptidyl aminopeptidase antibody
    • Tripeptidyl peptidase I antibody
    • Tripeptidyl-peptidase 1 antibody
    • Tripeptidyl-peptidase I antibody
    see all

画像

  • TPP1 antibody (ab54685) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.


  • Predicted band size : 61 kDa
  • Overlay histogram showing JEG3 cells stained with ab54685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54685, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Baradaran-Heravi A  et al. Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity. Proc Natl Acad Sci U S A 114:3479-3484 (2017). Read more (PubMed: 28289221) »
  • Baradaran-Heravi A  et al. Novel small molecules potentiate premature termination codon readthrough by aminoglycosides. Nucleic Acids Res 44:6583-98 (2016). Read more (PubMed: 27407112) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount
40 µg
Specification
Heart, Left Ventricle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dawn Bowles

Verified customer

投稿 Dec 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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