製品の概要

  • 製品名Anti-TPP1 antibody
    TPP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to TPP1
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 195-305 of Human TPP1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
  • 組織特異性Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
  • 関連疾患Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles.
  • 配列類似性Belongs to the peptidase S53 family.
  • 翻訳後修飾Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity.
  • 細胞内局在Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell growth inhibiting gene 1 protein antibody
    • Cell growth-inhibiting gene 1 protein antibody
    • Ceroid lipofuscinosis neuronal 2 antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile (Jansky Bielschowsky disease) antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile antibody
    • CLN 2 antibody
    • CLN2 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • Growth inhibiting protein 1 antibody
    • LPIC antibody
    • Lysosomal pepstatin insensitive protease antibody
    • Lysosomal pepstatin-insensitive protease antibody
    • MGC21297 antibody
    • TPP 1 antibody
    • TPP I antibody
    • TPP-1 antibody
    • TPP-I antibody
    • Tpp1 antibody
    • TPP1_HUMAN antibody
    • TPPI antibody
    • Tripeptidyl aminopeptidase antibody
    • Tripeptidyl peptidase I antibody
    • Tripeptidyl-peptidase 1 antibody
    • Tripeptidyl-peptidase I antibody
    see all

Anti-TPP1 antibody 画像

  • TPP1 antibody (ab54685) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.


  • Predicted band size : 61 kDa
  • Overlay histogram showing JEG3 cells stained with ab54685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54685, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-TPP1 antibody (ab54685) 使用論文

This product has been referenced in:
  • Baradaran-Heravi A  et al. Novel small molecules potentiate premature termination codon readthrough by aminoglycosides. Nucleic Acids Res 44:6583-98 (2016). Read more (PubMed: 27407112) »
  • Lojewski X  et al. Human iPSC models of neuronal ceroid lipofuscinosis capture distinct effects of TPP1 and CLN3 mutations on the endocytic pathway. Hum Mol Genet 23:2005-22 (2014). WB ; Human . Read more (PubMed: 24271013) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount 40 µg
Specification Heart, Left Ventricle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dawn Bowles

Verified customer

投稿 Dec 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"