製品の概要

  • 製品名Anti-TPOR antibody [EPR4194]
    TPOR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4194] to TPOR
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TPOR aa 600 to the C-terminus.
    Database link: P40238

  • ポジティブ・コントロール
    • HepG2, A431, and Human marrow lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名EPR4194
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.

ターゲット情報

  • 機能Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.
  • 組織特異性Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
  • 関連疾患Defects in MPL are a cause of congenital amegakaryocytic thrombocytopenia (CAMT) [MIM:604498]. CAMT is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C MPL antibody
    • CD110 antibody
    • mpl antibody
    • MPLV antibody
    • Myeloproliferative leukemia protein antibody
    • Myeloproliferative leukemia virus oncogene antibody
    • Proto-oncogene c-Mpl antibody
    • THCYT2 antibody
    • Thrombopoietin receptor antibody
    • TPO R antibody
    • TPO-R antibody
    • TPOR antibody
    • TPOR_HUMAN antibody
    see all

Anti-TPOR antibody [EPR4194] 画像

  • Anti-TPOR antibody [EPR4194] (ab109003) at 1/1000 dilution (purified) + mouse spleen lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 71 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-TPOR antibody [EPR4194] (ab109003) at 1/5000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 71 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-TPOR antibody [EPR4194] (ab109003) at 1/2500 dilution (purified) + A431 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 71 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-TPOR antibody [EPR4194] (ab109003) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Human marrow lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 71 kDa

Anti-TPOR antibody [EPR4194] (ab109003) 使用論文

ab109003 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109003.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"