製品の概要

  • 製品名Anti-TPMT antibody [AT2E7]
    TPMT 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AT2E7] to TPMT
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-245 of Human TPMT purified from E. coli (NP_000358).

  • ポジティブ・コントロール
    • Hep3B cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
  • 関連疾患Defects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.
  • 配列類似性Belongs to the methyltransferase superfamily. TPMT family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HGNC:12014 antibody
    • S adenosyl L methionine thiopurine S methyltransferase antibody
    • Thiopurine methyltransferase antibody
    • Thiopurine S methyltransferase antibody
    • Thiopurine S-methyltransferase antibody
    • TPMT antibody
    • TPMT_HUMAN antibody
    see all

Anti-TPMT antibody [AT2E7] 画像

  • Anti-TPMT antibody [AT2E7] (ab122984) at 1/1000 dilution + Hep3B lysate at 40 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 28 kDa


  • Predicted band size : 28 kDa

    The Recombinant Protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : Recombinant Protein
    Lane 2 : HepG2 cell lysate

  • Immunocytochemistry/Immunofluorescence analysis of U87MG cells labelling TPMT with ab122984 at 1/100. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Flow Cytometry analysis of U87MG cells labelling TPMT with ab122984, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.

Anti-TPMT antibody [AT2E7] (ab122984) 使用論文

ab122984 has not yet been referenced specifically in any publications.

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