製品の概要

  • 製品名Anti-TPH2 antibody
    TPH2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TPH2
  • アプリケーション適用あり: WB, IP, ICC, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human, Non Human Primates
  • 免疫原

    Synthetic peptide:

    RRGLSLDSAVPEDHQL

    , corresponding to N terminal amino acids 15-30 of Human TPH2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP 1/1000.
ICC 1/500.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 組織特異性Brain specific.
  • パスウェイAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • 関連疾患Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
  • 配列類似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC138871 antibody
    • ADHD7 antibody
    • FLJ37295 antibody
    • MGC138872 antibody
    • Neuronal tryptophan hydroxylase antibody
    • NTPH antibody
    • TPH 2 antibody
    • Tph2 antibody
    • TPH2_HUMAN antibody
    • Tryptophan 5-hydroxylase 2 antibody
    • Tryptophan 5-monooxygenase 2 antibody
    • Tryptophan hydroxylase 2 antibody
    see all

Anti-TPH2 antibody 画像

  • Immunoprecipitation analysis of Raphe Nuclei isolated from Sprague-Dawley, precipitating ab111828 coupled to magnetic beads, which was introduced to whole cell extracts at room temperature for 15 mins. Immunoprecipitated proteins were assessed by Western blotting.

     

    Lane 1 (Positive Control): Non-immunoprecipitated brain lysates were probed with ab111828.
    Lane 2 (ab111828 Ms TPH2): Immunoprecipitated Proteins
    Lane 3 (Sheep TPH2 antibody): Immunoprecipitated Proteins
    Lane 4 (Negative Control => Anti-Tyrosine Hydroxylase antibody): Immunoprecipitated proteins

  • ab111828 staining TPH2 in Mouse dorsal raphe coronal brain slices tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 2% serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/800 in PBS + Triton X-100 + serum) at 22°C for 24 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500 in PBS + Goat serum) was used as the secondary antibody.

    See Abreview

Anti-TPH2 antibody (ab111828) 使用論文

ab111828 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (Dorsal Raphe coronal brain slices)
Specification Dorsal Raphe coronal brain slices
Permeabilization Yes - TritonX-100, 0.3%
Fixative Paraformaldehyde
Username

Jason Teem

Verified customer

投稿 Nov 04 2013

DISCOUNT CODE: *** Expiration date: April 21st, 2012 I am very pleased to hear you would like to use ab111828 in IHC and send us an image of your results. This code will give you 1 free primary antibody before the expiration date. To redeem this offer,...

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Thank you very much for your interest in ab111828. Unfortunately, we would be unable to ship this antibody faster than 2-3 weeks, and we do not currently have an image of ab111828 in IHC. Therefore, I can offer a discount off a future purchase if you b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"