Anti-TPCN1 抗体 (ab94731)

製品の概要

  • 製品名Anti-TPCN1 antibody
  • 製品の詳細
    Rabbit polyclonal to TPCN1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (QEAAIYLQEG ENNDKFFTHP KDAKALAAYL FAHNHLFYLM ELATALLLLL) of Human TPCN1 (NP_060371).

  • ポジティブ・コントロール
    • HT1080 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal and endosomal membrane.
  • 組織特異性Highest expression found in the heart and kidney, and lowest expression found in the spleen.
  • 配列類似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
  • ドメインEach of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • 細胞内局在Lysosome membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ20612 antibody
    • KIAA1169 antibody
    • TPC1 antibody
    • TPC1_HUMAN antibody
    • TPCN 1 antibody
    • TPCN1 antibody
    • Two pore calcium channel protein 1 antibody
    • Two pore channel 1 antibody
    • Two pore segment channel 1 antibody
    • Voltage dependent calcium channel protein TPC1 antibody
    • Voltage-dependent calcium channel protein TPC1 antibody
    see all

Anti-TPCN1 antibody 画像

  • Anti-TPCN1 antibody (ab94731) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa
    Gel concentration: 6-18%
  • ab94731 staining TPCN1 in Zebrafish 1000-cell embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in BSA in PBS-T) for 18 hours at 4°C. An AlexaFluor® 488-conjugated Goat-anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 94 kDa
    Western Blot using ab94731 at 1.0ug/ml. Sample type: Human HT1080 cell Lysate

Anti-TPCN1 antibody (ab94731) 使用論文

ab94731 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (1000-cell embryo)
Specification 1000-cell embryo
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Celeste Eno

Verified customer

投稿 Jun 19 2012

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