Anti-TPA Tissue Plasminogen Activator 抗体 (Biotin) (ab28208)
製品の概要
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製品名Anti-TPA Tissue Plasminogen Activator antibody (Biotin)
TPA Tissue Plasminogen Activator 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TPA Tissue Plasminogen Activator (Biotin)
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由来種Rabbit
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標識Biotin
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Mouse
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免疫原
Recombinant full length protein (Mouse)
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ポジティブ･コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal kidney.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA. pH 7.4 -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab28208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 5 µg/ml. Predicted molecular weight: 63 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
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組織特異性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
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関連疾患Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
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配列類似性Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
ドメインBoth FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
翻訳後修飾The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
細胞内局在Secreted > extracellular space.
- Information by UniProt
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参照データベース
- Entrez Gene: 18791 Mouse
- SwissProt: P11214 Mouse
- Unigene: 154660 Mouse
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別名
- Alteplase antibody
- DKFZp686I03148 antibody
- Plasminogen activator tissue antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPA Tissue Plasminogen Activator antibody (Biotin) (ab28208)IHC image of TPA Tissue Plasminogen Activator staining in Mouse normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28208, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
This product has been referenced in:
- Li QK et al. Serum fucosylated prostate-specific antigen (PSA) improves the differentiation of aggressive from non-aggressive prostate cancers. Theranostics 5:267-76 (2015). Read more (PubMed: 25553114) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"