Anti-TPA Tissue Plasminogen Activator 抗体 (ab28219)

製品の概要

  • 製品名Anti-TPA Tissue Plasminogen Activator antibody
    TPA Tissue Plasminogen Activator 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TPA Tissue Plasminogen Activator
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length TPA Tissue Plasminogen Activator protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
ELISA 1/100000.

ターゲット情報

  • 機能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 組織特異性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 関連疾患Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • ドメインBoth FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻訳後修飾The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alteplase antibody
    • DKFZp686I03148 antibody
    • Plasminogen activator tissue antibody
    • Plasminogen activator tissue type antibody
    • PLAT antibody
    • Reteplase antibody
    • t PA antibody
    • T Plasminogen Activator antibody
    • t-PA antibody
    • T-plasminogen activator antibody
    • Tissue plasminogen activator (t PA) antibody
    • Tissue type plasminogen activator antibody
    • Tissue-type plasminogen activator chain B antibody
    • tPA antibody
    • TPA_HUMAN antibody
    • TPA1 antibody
    see all

Anti-TPA Tissue Plasminogen Activator antibody (ab28219) 使用論文

This product has been referenced in:
  • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »

See 1 Publication for this product

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Thank you for contacting us.

This antibody has not been purified and therefore, its concentration has not been determined. However we estimate that its concentration will be in the region of 1-10 mg/ml.

I hope this information is ...

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I do have a few recommendations that might help. One thing you can do is block the membranes overnight at four degrees Celsius which can help reduce background. Also, incubating the primary antibody with blocking solution that also has 0.01% tween 20 c...

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Thank you for contacting Abcam. I am sorry for the confusion about the storage conditions. I think the antibodies will most likely be fine if you can aliquot them and get them in a freezer. We have done several tests with the stability of our antibodie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"