Total OXPHOS Human WB 抗体 Cocktail (ab110411)

製品の概要

  • 製品名
    Total OXPHOS Human WB Antibody Cocktail
  • アッセイタイプ
    Quantitative
  • 種交差性
    交差種: Human
  • 製品の概要

    ab110411  contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.


    Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.


    The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).


    The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.


    The five antibodies provided in the cocktail are all mouse monoclonal antibodies.


    We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.


    Due to a policy change, the previously provided control for this kit has been removed.

  • アプリケーション
    適用あり: WBmore details

製品の特性

  • 別名
    • OXPHOS cocktail

アプリケーション

Our Abpromise guarantee covers the use of ab110411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.

Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user.

We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.

Total OXPHOS Human WB Antibody Cocktail 画像

  • This image is courtesy of an anonymous Abreview

    Gel: Non-reduced Denaturing (4-12%)

    Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.

    Blocked for 1 hour with 5% Milk at room temperature.

    ab110411 used as per instructions.

    Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300

    Detection method: ECL+ 2 minutes exposure.

     

  • Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts.
    ab110411(MS601) was used to label lane 6, and the individual antibodies
    comprising the cocktail were used for lanes 1-5.

Total OXPHOS Human WB Antibody Cocktail (ab110411) 使用論文

This product has been referenced in:
  • Nafisinia M  et al. Whole Exome Sequencing Identifies the Genetic Basis of Late-Onset Leigh Syndrome in a Patient with MRI but Little Biochemical Evidence of a Mitochondrial Disorder. JIMD Rep 32:117-124 (2017). WB ; Human . Read more (PubMed: 27344648) »
  • Bhattacharya D  et al. Decreased transcriptional corepressor p107 is associated with exercise-induced mitochondrial biogenesis in human skeletal muscle. Physiol Rep 5:N/A (2017). WB ; Human . Read more (PubMed: 28270591) »

See all 121 Publications for this product

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Western

Excellent Excellent 5/5 (Ease of Use)
Abreviews
MitoProfile Total OXPHOS
40 ug total protein from human heart tissue whole lysate
12% tris-glycine gel
5% milk-TBST block, 1 h, RT
primary of 1:750 in 5% milk for 16 h at 4C
secondary of 1:1250 in 5% milk for 1 h at RT
exposure varied based on band
excellent ease of use - 5/5
image attached
Username

Abcam user community

Verified customer

投稿 Oct 29 2014

Total OXPHOS antibody for western blotting

Excellent Excellent 5/5 (Ease of Use)
Abreviews
human fibroblast whole lysate used to detect OXPHOS
Username

Abcam user community

Verified customer

投稿 Jul 10 2017

Total OXPHOS antibody cocktail

Excellent Excellent 5/5 (Ease of Use)
Abreviews
In this experiment we used isolated mitochondria from human HCT116 colon cancer cell line. The proteins from the mitochondrial lysate were seperated on a 13% SDS gel. The total OXPHOS human WB antibody kit was diluted 1:1000 in 2% milk. We already obtained strong signals of the complexes V and I after 10 sec. After 1 min (attached figure) we got signals from all five complexes.

The kit is easy to use and gives reproducibly results.
Username

Mr. Christian Marx

Verified customer

投稿 Aug 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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