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ab110411 contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).
The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.
The five antibodies provided in the cocktail are all mouse monoclonal antibodies.
We recommend using a high pH CAPS buffer / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.
Due to a policy change, the previously provided control for this kit has been removed.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
|Premix Cocktail of 5 monoclonal antibodies||1 x 720µg|
Our Abpromise guarantee covers the use of ab110411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user.
We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.
Gel: Non-reduced Denaturing (4-12%)
Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.
Blocked for 1 hour with 5% Milk at room temperature.
ab110411 used as per instructions.
Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300
Detection method: ECL+ 2 minutes exposure.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"