Anti-Torsin A/DYT1 抗体 [EP2569Y] (ab76133)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2569Y] to Torsin A/DYT1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Torsin A/DYT1 antibody [EP2569Y]
Torsin A/DYT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2569Y] to Torsin A/DYT1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Torsin A/DYT1 aa 300-400 (C terminal). The exact sequence is proprietary.
-
ポジティブ・コントロール
- WB: SW480, Caco-2, A549, NIH/3T3, and C6 whole cell lysates. Rat brain and mouse lung tissue lysates; Flow Cyt (intra): SH-SY5Y cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2569Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76133の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100.
For unpurified used at 1/150 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
|
特記事項 |
---|
Flow Cyt (Intra)
1/100. For unpurified used at 1/150 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
- Information by UniProt
-
参照データベース
- Entrez Gene: 1861 Human
- Entrez Gene: 30931 Mouse
- Entrez Gene: 266606 Rat
- Omim: 605204 Human
- SwissProt: O14656 Human
- SwissProt: Q9ER39 Mouse
- Unigene: 534312 Human
- Unigene: 154994 Mouse
-
別名
- DQ2 antibody
- Dystonia 1 antibody
- Dystonia 1 protein antibody
see all
画像
-
All lanes : Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133) at 1/5000 dilution (purified)
Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 6 : Mouse lung lysate
Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab76133 は論文での使用が確認できていません。