Anti-TORC2 抗体 (ab115035)

製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to TORC2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-HGQQPYHRPIND

    , corresponding to internal sequence amino acids 541-552 of Mouse Torc2.

  • ポジティブ・コントロール
    • Mouse and Rat brain and spinal cord lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).

ターゲット情報

  • 機能
    Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
  • 組織特異性
    Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
  • 配列類似性
    Belongs to the TORC family.
  • 翻訳後修飾
    Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CREB regulated transcription coactivator 2 antibody
    • CREB-regulated transcription coactivator 2 antibody
    • CRTC2 antibody
    • CRTC2_HUMAN antibody
    • RP11-422P24.6 antibody
    • TORC-2 antibody
    • torc2 antibody
    • Transducer of CREB protein 2 antibody
    • Transducer of regulated cAMP response element-binding protein antibody
    • Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody
    • Transducer of regulated cAMP response element-binding protein 2 antibody
    • Transducer of regulated CREB protein 2 antibody
    see all

画像

  • Anti-TORC2 antibody (ab115035) at 0.03 µg/ml + Rat spinal cord lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab115035 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (LIver)
Specification
LIver
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Verified customer

投稿 Feb 03 2012

Thank you for your reply. We would suggest that procedures will usually require some optimization by the end user. However, I am pleased to provide the following suggestions: For how long can I keep the fixed coverslips before the block...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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