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RabMAb

Anti-TORC1 抗体 [EPR3381(2)] (ab109736)

製品の概要

  • 製品名
    Anti-TORC1 antibody [EPR3381(2)]
    TORC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3381(2)] to TORC1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human MECT1.

  • ポジティブ・コントロール
    • 293T, Human fetal brain, and HeLa lysates; Human brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能
      Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
    • 組織特異性
      Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
    • 関連疾患
      A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
    • 配列類似性
      Belongs to the TORC family.
    • 翻訳後修飾
      Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
    • 細胞内局在
      Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
    • Information by UniProt
    • 参照データベース
    • 別名
      • KIAA0616 antibody
      • CREB regulated transcription coactivator 1 antibody
      • CREB-regulated transcription coactivator 1 antibody
      • CRTC1 antibody
      • CRTC1_HUMAN antibody
      • FLJ14027 antibody
      • MECT 1 antibody
      • Mucoepidermoid carcinoma translocated 1 antibody
      • Mucoepidermoid carcinoma translocated protein 1 antibody
      • TORC-1 antibody
      • TORC1 antibody
      • Transducer of CREB protein 1 antibody
      • Transducer of regulated cAMP response element binding protein 1 antibody
      • Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody
      • Transducer of regulated cAMP response element-binding protein 1 antibody
      • WAMTP1 antibody
      see all

    画像

    • All lanes : Anti-TORC1 antibody [EPR3381(2)] (ab109736) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 67 kDa
    • ab109736 at 1/50 dilution staining MECT1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    参考文献

    ab109736 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for your enquiry and your interest in our products.

    We are not aware if any of our antibodies to TORC1 (CRTC1) have been involved in such scientific investigations. If they were, then the publication list of the on-line datasheet wo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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