製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TORC1
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 25 and 75 of human MECT1 (NP_056136.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
  • 追加情報
    IP: Use at 2-5µg/mg of lysate.


    Is unsuitable for WB.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
    • 組織特異性
      Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
    • 関連疾患
      A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
    • 配列類似性
      Belongs to the TORC family.
    • 翻訳後修飾
      Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
    • 細胞内局在
      Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
    • Information by UniProt
    • 参照データベース
    • 別名
      • KIAA0616 antibody
      • CREB regulated transcription coactivator 1 antibody
      • CREB-regulated transcription coactivator 1 antibody
      • CRTC1 antibody
      • CRTC1_HUMAN antibody
      • FLJ14027 antibody
      • MECT 1 antibody
      • Mucoepidermoid carcinoma translocated 1 antibody
      • Mucoepidermoid carcinoma translocated protein 1 antibody
      • TORC-1 antibody
      • TORC1 antibody
      • Transducer of CREB protein 1 antibody
      • Transducer of regulated cAMP response element binding protein 1 antibody
      • Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody
      • Transducer of regulated cAMP response element-binding protein 1 antibody
      • WAMTP1 antibody
      see all

    プロトコール

    参考文献

    ab70823 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (30 ug of WCL was resolved on a 10% PAGE gel)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE gel)
    Loading amount
    30 µg
    Treatment
    100 nm MLN0128 (TORC1 inhibitor)
    Specification
    30 ug of WCL was resolved on a 10% PAGE gel
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Nov 30 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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