All lanes : Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa Observed band size: 66 kDa
Exposure time: 30 seconds
Immunoprecipitation - TOR1AIP1 antibody (ab86307)
Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.