Anti-Topoisomerase II beta 抗体 [EPR5377] (ab109524)




Our Abpromise guarantee covers the use of ab109524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 183 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • 配列類似性Belongs to the type II topoisomerase family.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm. Nucleus > nucleolus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Antigen MLAA 44 antibody
      • beta isozyme antibody
      • DNA topoisomerase 2 beta antibody
      • DNA topoisomerase 2-beta antibody
      • DNA topoisomerase II 180 kD antibody
      • DNA topoisomerase II antibody
      • DNA topoisomerase II beta antibody
      • DNA topoisomerase II beta isozyme antibody
      • Top 2 antibody
      • TOP 2B antibody
      • TOP IIB antibody
      • Top2 beta antibody
      • TOP2B antibody
      • TOP2B_HUMAN antibody
      • Top2beta antibody
      • TOPIIB antibody
      • Topo II beta antibody
      • Topoisomerase (DNA) II beta 180kDa antibody
      • Topoisomerase (DNA) II beta antibody
      • Topoisomerase IIb antibody
      • U937 associated antigen antibody
      see all

    Anti-Topoisomerase II beta antibody [EPR5377] 画像

    • ICC/IF image of ab109524 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109524, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-Topoisomerase II beta antibody [EPR5377] (ab109524) at 1/50000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Raw264.7 cell lysate
      Lane 4 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 183 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    • ab109524, at a 1/100 dilution, staining Human Topoisomerase II beta in breast tissue, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-Topoisomerase II beta antibody [EPR5377] (ab109524) 使用論文

    This product has been referenced in:
    • Kouzine F  et al. Transcription-dependent dynamic supercoiling is a short-range genomic force. Nat Struct Mol Biol 20:396-403 (2013). Human . Read more (PubMed: 23416947) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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    投稿 Jun 20 2014