Anti-Topoisomerase II beta 抗体 (ab52769)

製品の概要

  • 製品名Anti-Topoisomerase II beta antibody
    Topoisomerase II beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Topoisomerase II beta
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Chicken
    交差が予測される動物種: Mouse, Rat, Dog, Human, Chimpanzee
  • 免疫原

    Synthetic peptide: LVNAADNKQR DKNMTCIKIS IDPESNIISI WNNGKGIPVV EHKVEKVYVP ALIFGQLLTS SNYDDDEKKV TGGRNGYGAK LCNIFSTKFT VETACKEYKH , corresponding to amino acids 115-215 of Chicken Topoisomerase II beta; which has been tagged

  • 特記事項

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 183 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 配列類似性Belongs to the type II topoisomerase family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen MLAA 44 antibody
    • beta isozyme antibody
    • DNA topoisomerase 2 beta antibody
    • DNA topoisomerase 2-beta antibody
    • DNA topoisomerase II 180 kD antibody
    • DNA topoisomerase II antibody
    • DNA topoisomerase II beta antibody
    • DNA topoisomerase II beta isozyme antibody
    • Top 2 antibody
    • TOP 2B antibody
    • TOP IIB antibody
    • Top2 beta antibody
    • TOP2B antibody
    • TOP2B_HUMAN antibody
    • Top2beta antibody
    • TOPIIB antibody
    • Topo II beta antibody
    • Topoisomerase (DNA) II beta 180kDa antibody
    • Topoisomerase (DNA) II beta antibody
    • Topoisomerase IIb antibody
    • U937 associated antigen antibody
    see all

Anti-Topoisomerase II beta antibody 画像

  • All lanes : Anti-Topoisomerase II beta antibody (ab52769) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 183 kDa

Anti-Topoisomerase II beta antibody (ab52769) 使用論文

ab52769 has not yet been referenced specifically in any publications.

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