Anti-Topoisomerase II alpha (phospho T1343) 抗体 [EP1054Y] (ab52853)

製品の概要

  • 製品名Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
    Topoisomerase II alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • 特異性ab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
  • アプリケーション適用あり: IHC-P, WB, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.

  • ポジティブ・コントロール
    • WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • 配列類似性Belongs to the type II topoisomerase family.
    • 翻訳後修飾Phosphorylation has no effect on catalytic activity.
    • 細胞内局在Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] 画像

    • All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with AP
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 174 kDa
      Observed band size : 174 kDa
    • ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) 使用論文

    ab52853 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"