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RabMAb

Anti-Topoisomerase II alpha (phospho T1343) 抗体 [EP1054Y] (ab52853)

製品の概要

  • 製品名
    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
    Topoisomerase II alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • 特異性
    ab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
  • アプリケーション
    適用あり: IHC-P, WB, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.

  • ポジティブ・コントロール
    • WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC 1/50 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • 配列類似性
      Belongs to the type II topoisomerase family.
    • 翻訳後修飾
      Phosphorylation has no effect on catalytic activity.
    • 細胞内局在
      Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    画像

    • All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with AP
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 174 kDa
      Observed band size : 174 kDa
    • ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

    参考文献

    ab52853 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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