Rabbit monoclonal [EP2722] to Topoisomerase II alpha (phospho S1106)
ab75765 detects Topoisomerase II alpha phosphorylated on Serine 1106.
A phospho specific peptide corresponding to residues surrounding Serine 1106 of human Topoisormerase II alpha
HeLa cell lysates, untreated or treated with AP and colonic
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Belongs to the type II topoisomerase family.
Phosphorylation has no effect on catalytic activity.
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
Information by UniProt
alpha isozyme antibody
ATP hydrolyzing DNA topoisomerase II alfa antibody
DNA gyrase antibody
Western blot - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
All lanes : Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilution Lane 1 : HeLa cell lysates, untreated Lane 2 : HeLa cell lysates, treated with AP Lysates/proteins at 10 µg per lane. Secondary All lanes : goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 174 kDa Observed band size: 174 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
ab75765 at 1/100 dilution staining Topoisomerase II alpha in colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue
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