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RabMAb

Anti-Topoisomerase II alpha 抗体 [EP1102Y] (ab52934)

製品の概要

  • 製品名
    Anti-Topoisomerase II alpha antibody [EP1102Y]
    Topoisomerase II alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1102Y] to Topoisomerase II alpha
  • 特異性
    Please be aware that the antibody provided positive results in cell lysate samples but negative results in tissue lysate samples.
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Topoisomerase II alpha (C terminal).

  • ポジティブ・コントロール
    • HeLa cell lysate; human breast carcinoma tissue. WB: Hela, Jurkat, Neuro-2a and PC-12 whole cell lysate; Human breast cancer, Mouse thymus lysate, Rat thymus lysate and Rat testis lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • 配列類似性
      Belongs to the type II topoisomerase family.
    • 翻訳後修飾
      Phosphorylation has no effect on catalytic activity.
    • 細胞内局在
      Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    画像

    • All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
      Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
      Lane 3 : Neuro-2a (mouse neuroblastoma) whole cell lysate
      Lane 4 : Human breast cancer lysate
      Lane 5 : Mouse thymus lysate
      Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 7 : Rat thymus lysate
      Lane 8 : Rat testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 174 kDa
      Observed band size : 174 kDa

      Exposure time:

      Lanes 1-3: 1 second.
      Lanes 4-7: 3 minutes.
      Lane 8: 7 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Scapulatempo-Neto C  et al. Characterization of topoisomerase II a and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13:1891-1898 (2017). IHC-P ; Human . Read more (PubMed: 28454340) »
    • Guturi KK  et al. RNF168 and USP10 regulate topoisomerase IIa function via opposing effects on its ubiquitylation. Nat Commun 7:12638 (2016). Read more (PubMed: 27558965) »

    See all 11 Publications for this product

    レビューと Q&A

    DISCOUNT CODE: ****** Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab52934 in ChIP]. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abre...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon ...)
    Specification
    Colon ...
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from DAKO
    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 Mar 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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