Anti-Topoisomerase II alpha 抗体 (ab12318)

製品の概要

  • 製品名Anti-Topoisomerase II alpha antibody
    Topoisomerase II alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Topoisomerase II alpha
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide representing a portion of human DNA topoisomerase II, alpha isozyme encoded within exon 35 (LocusLink ID 7153).

  • ポジティブ・コントロール
    • HeLa cells. IHC-P: FFPE human testis tissue sections.
  • 特記事項Centrifuge tube to remove product from lid.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/30000. Detects a band of approximately 178 kDa (predicted molecular weight: 184 kDa).
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • 配列類似性Belongs to the type II topoisomerase family.
  • 翻訳後修飾Phosphorylation has no effect on catalytic activity.
  • 細胞内局在Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha isozyme antibody
    • ATP hydrolyzing DNA topoisomerase II alfa antibody
    • DNA gyrase antibody
    • DNA topoisomerase (ATP hydrolyzing) antibody
    • DNA topoisomerase 2 alpha antibody
    • DNA topoisomerase 2-alpha antibody
    • DNA topoisomerase II 170 kD antibody
    • DNA topoisomerase II alpha isozyme antibody
    • DNA topoisomerase II antibody
    • DNA Topoisomerase2 antibody
    • TOP 2A antibody
    • TOP2 antibody
    • TOP2A antibody
    • TOP2A_HUMAN antibody
    • Topoisomerase DNA II alpha 170kDa antibody
    • TP2A antibody
    see all

Anti-Topoisomerase II alpha antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.
  • IHC image of Topoisomerase II alpha staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12318, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.

    Samples: Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).

Anti-Topoisomerase II alpha antibody (ab12318) 使用論文

This product has been referenced in:
  • Gavrilov AA  et al. Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub. Nucleic Acids Res 41:3563-75 (2013). ICC/IF ; Mouse . Read more (PubMed: 23396278) »
  • Nakamura M  et al. Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR. Retrovirology 9:13 (2012). Human . Read more (PubMed: 22316138) »

See all 4 Publications for this product

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Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount 90 µg
Specification embryonic fibroblasts
Treatment 250 uM etoposide for 4h, 1 uM camptothecin for 1h
Gel Running Conditions Reduced Denaturing (4-18% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"