Anti-Topoisomerase I 抗体 [Mab1] (ab12407)

製品の概要

  • 製品名Anti-Topoisomerase I antibody [Mab1]
    Topoisomerase I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Mab1] to Topoisomerase I
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human).

  • ポジティブ・コントロール
    • Extract of A231 human kidney cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名Mab1
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab12407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).

ターゲット情報

  • 機能The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • 関連疾患Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • 配列類似性Belongs to the eukaryotic type I topoisomerase family.
  • 翻訳後修飾Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • 細胞内局在Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Anti-Topoisomerase I antibody [Mab1] (ab12407) 使用論文

ab12407 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and for providing more details of your protocol. This antibody has been tested for Western blot application using A231 human kidney cells. Thus, we would suggest running a positive control along with your samples. As w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"