Anti-Topoisomerase I 抗体 [EPR5375] (ab109374)

製品の概要

  • 製品名Anti-Topoisomerase I antibody [EPR5375]
    Topoisomerase I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5375] to Topoisomerase I
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the N-terminus of Human Topoisomerase I

  • ポジティブ・コントロール
    • Jurkat, SW480, and K562 cell lysates, Human breast carcinoma and Human colonic carcinoma tissues, MCF7 cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Topoisomerase I antibody (Alexa Fluor® 488) [EPR5375] (ab197505)
    Anti-Topoisomerase I antibody (Alexa Fluor® 647) [EPR5375] (ab196930)
    Anti-Topoisomerase I antibody (HRP) [EPR5375] (ab196642)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 91 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
    • 関連疾患Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
    • 配列類似性Belongs to the eukaryotic type I topoisomerase family.
    • 翻訳後修飾Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
    • 細胞内局在Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DNA topoisomerase 1 antibody
      • DNA topoisomerase I antibody
      • NUP98 fusion gene antibody
      • TOP 1 antibody
      • TOP I antibody
      • TOP1 antibody
      • TOP1_HUMAN antibody
      • TOPI antibody
      • Topoisomerase (DNA) I antibody
      • Topoisomerase 1 antibody
      • Topoisomerase1 antibody
      • TopoisomeraseI antibody
      • Type I DNA topoisomerase antibody
      see all

    Anti-Topoisomerase I antibody [EPR5375] 画像

    • Overlay histogram showing HepG2 cells stained with ab109374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109374, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Topoisomerase I antibody [EPR5375] (ab109374) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 91 kDa
    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109374 at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue using ab109374 at a dilution of 1/100.
    • Immunofluorescent staining of MCF7 cells using ab109374 at a dilution of 1/100.

    Anti-Topoisomerase I antibody [EPR5375] (ab109374) 使用論文

    This product has been referenced in:
    • Groh M  et al. R-loops associated with triplet repeat expansions promote gene silencing in Friedreich ataxia and fragile X syndrome. PLoS Genet 10:e1004318 (2014). WB . Read more (PubMed: 24787137) »
    • Mamouni K  et al. RhoB promotes ?H2AX dephosphorylation and DNA double-strand break repair. Mol Cell Biol 34:3144-55 (2014). Read more (PubMed: 24912678) »

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
    Loading amount 50 µg
    Specification U2OS cells
    Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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    Verified customer

    投稿 Oct 05 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (fibroblast)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 40 µg
    Specification fibroblast
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    投稿 Aug 05 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (fibroblast)
    Permeabilization Yes - block buffer contains 0.3% TritonX-100, 1H, RT
    Specification fibroblast
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

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    Verified customer

    投稿 Jun 29 2016

    Merci de nous avoir contactés.

    La concentration de l'anti-Topoisomerase I ab109374, lot numéro GR49853-3 est 0.109 mg/mL.

    J'espère que cette information vous sera utile. N'hésitez pas à nous contac...

    Read More

    Thank you for your enquiry. Please find enclosed the two requested CoC for ab3380 (Lot NO.:GR56451-1) and ab109374 (Lot NO.:GR49853-2) as attachments. I hope this helps and if I can assist further, please do not hesitate to contact me.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"