Anti-TOM70 抗体 (ab89624)
Key features and details
- Mouse polyclonal to TOM70
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TOM70 antibody
TOM70 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to TOM70 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human TOM70. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_055635).
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ポジティブ・コントロール
- Human liver tissue lysate, HepG2 whole cell lysate (ab7900) and TOM70 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89624の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. |
ターゲット情報
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機能
Receptor that accelerates the import of all mitochondrial precursor proteins. -
配列類似性
Belongs to the Tom70 family.
Contains 10 TPR repeats. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9868 Human
- Omim: 606081 Human
- SwissProt: O94826 Human
- Unigene: 227253 Human
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別名
- FLJ9047 antibody
- Mitochondrial import receptor subunit TOM70 antibody
- Mitochondrial precursor proteins import receptor antibody
see all
画像
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Anti-TOM70 antibody (ab89624) at 1 µg/ml + human liver tissue lysate at 50 µg
Predicted band size: 67 kDa
Observed band size: 67 kDa -
Anti-TOM70 antibody (ab89624) at 1 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TOM70 antibody (ab89624) at 1 µg/ml
Lane 1 : TOMM70A transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (4)
ab89624 は 4 報の論文で使用されています。
- Richter M et al. The Recombinant Fragment of Human ?-Casein Induces Cell Death by Targeting the Proteins of Mitochondrial Import in Breast Cancer Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32486420
- Reyes A et al. RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. PLoS Genet 16:e1008923 (2020). PubMed: 32735630
- Wohlfromm F et al. Interplay Between Mitophagy and Apoptosis Defines a Cell Fate Upon Co-treatment of Breast Cancer Cells With a Recombinant Fragment of Human ?-Casein and Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand. Front Cell Dev Biol 8:617762 (2020). PubMed: 33537307
- Rakovic A et al. Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1)-dependent ubiquitination of endogenous Parkin attenuates mitophagy: study in human primary fibroblasts and induced pluripotent stem cell-derived neurons. J Biol Chem 288:2223-37 (2013). WB ; Human . PubMed: 23212910