Anti-TOMM70A 抗体 (ab83841)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TOMM70A
  • 特異性
    At least two isoforms of TOM70 are known to exist; this antibody will only detect the larger isoform.
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human TOMM70A (N terminal). ab83841 was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOM70.
    Database link: O94826

  • ポジティブ・コントロール
    • 293 cell lysate. Mouse brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2.5 - 20 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 67 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

画像

  • Lane 1 : Anti-TOMM70A antibody (ab83841) at 0.5 µg/ml
    Lane 2 : Anti-TOMM70A antibody (ab83841) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • Immunohistochemistry (IHC-P) of TOM70A in mouse brain tissue with ab83841 at 2.5 μg/ml.

  • Anti-TOMM70A antibody (ab83841) at 2 µg/ml + 293 cell lysate at 15 µg

    Predicted band size : 67 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of mouse brain tissue section labelling TOM70 with ab83841 at 5 ug/ml.



参考文献

ab83841 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Western blot
Sample
Rat Cell lysate - other (Whole cell lysate, and mitochondria isolated from)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Whole cell lysate, and mitochondria isolated from
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Svitlana Yablonska

Verified customer

投稿 Jun 21 2017

Thank you for contacting us.

Looking at the sequence alignment between the Human TOMM70A and the yeast TOMM70A (see attachment), I'm afraid that none of the anti-TOMM70A we have in our catalogseems to besuitable for yeast.

I am so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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