製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TOMM34
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-309 of Human TOMM34 (NP_006800.2).

  • ポジティブ・コントロール
    • Mouse testis tissue lysate. Hela NE.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.

ターゲット情報

  • 機能
    Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the Tom34 family.
    Contains 6 TPR repeats.
  • 細胞内局在
    Cytoplasm. Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hTom34 antibody
    • HTOM34P antibody
    • Mitochondrial import receptor subunit TOM34 antibody
    • OTTHUMP00000031079 antibody
    • Outer mitochondrial membrane translocase (34kD) antibody
    • TOM 34 antibody
    • TOM34 antibody
    • TOM34_HUMAN antibody
    • TOMM 34 antibody
    • TOMM34 antibody
    • Translocase of outer membrane 34 kDa subunit antibody
    • Translocase of outer mitochondrial membrane 34 antibody
    • URCC 3 antibody
    • URCC3 antibody
    see all

画像

  • Anti-TOMM34 antibody (ab103585) at 1/500 dilution + Mouse testis tissue lysate at 50 µg

    Predicted band size : 35 kDa
  • All lanes : Anti-TOMM34 antibody (ab103585) at 1/500 dilution

    Lane 1 : TOMM34 transfected 293T cell lysate
    Lane 2 : 293T non-transfected cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa
  • Anti-TOMM34 antibody (ab103585) at 1/500 dilution + HeLa NE at 50 µg

    Predicted band size : 35 kDa

プロトコール

参考文献

ab103585 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Aug 29 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Jul 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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