製品の概要

  • 製品名Anti-TOM1 antibody
    TOM1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to TOM1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: GLAGALDARQ QSTGAIPVTQ ACLMEDIEQW LSTDVGNDAE EPKGVTSEEF DKFLEERAKA ADRLPNLSSP SAEGPPGPPS GPAPRKKTQE KDDDMLFA, corresponding to amino acids 394-491 of Human TOM1 (NP_005479) with a proprietory tag.

  • ポジティブ・コントロール
    • HeLa cell lysate. Recombinant tagged human TOM1 fragment.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged TOM1 is approximately 30ng/ml when used as a capture antibody.

ターゲット情報

Anti-TOM1 antibody 画像

  • Anti-TOM1 antibody (ab77649) at 1 µg/ml + Recombinant tagged human TOM1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 54 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-TOM1 antibody (ab77649) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

Anti-TOM1 antibody (ab77649) 使用論文

ab77649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"