製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Tollip
  • アプリケーション
    適用あり: ELISA, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid peptide from near the center of human Tollip

  • ポジティブ・コントロール
    • Rat brain cell lysate and THP-1 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

ターゲット情報

Anti-Tollip antibody 画像

  • ab62578 at 2µg/ml staining Tollip in THP-1 cells by Immunocytochemistry.
  • Lane 1 : Anti-Tollip antibody (ab62578) at 0.5 µg/ml
    Lane 2 : Anti-Tollip antibody (ab62578) at 1 µg/ml
    Lane 3 : Anti-Tollip antibody (ab62578) at 2 µg/ml

    Lane 1 : rat brain cell lysate
    Lane 2 : rat brain cell lysate
    Lane 3 : rat brain cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa

Anti-Tollip antibody (ab62578) 使用論文

This product has been referenced in:
  • Sugi Y  et al. Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium. PLoS One 11:e0164858 (2016). WB ; Mouse . Read more (PubMed: 27741296) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Sample
Rat Cell lysate - whole cell (rat brain)
Specification
rat brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Aug 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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