The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 30 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
Belongs to the tollip family. Contains 1 C2 domain. Contains 1 CUE domain.
Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
ab115559, at 5µg/ml, staining Tollip in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.