製品の概要

  • 製品名Anti-TOB antibody
    TOB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TOB
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Chicken, Cow, Dog, Human, Pig
  • 免疫原

    A region within synthetic peptide: TFAATKFGST KMKNSGRSSK VARTSPINLG LTVNVNDLLK QKAISSSVHS, corresponding to internal sequence amino acids 181-230 of Human TOB

  • ポジティブ・コントロール
    • NIH 3T3 lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab48826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性The Tob/Btg family of proteins consists of a large number of members. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types. The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.
  • 参照データベース
  • 別名
    • APRO 6 antibody
    • APRO6 antibody
    • MGC104792 antibody
    • MGC34446 antibody
    • PIG 49 antibody
    • PIG49 antibody
    • Proliferation inducing gene 49 antibody
    • Protein Tob1 antibody
    • RP23 244C22.1 antibody
    • TOB 1 antibody
    • Tob 1 protein antibody
    • TOB antibody
    • TOB1 antibody
    • Tob1 protein antibody
    • Transducer of ERBB 2 1 antibody
    • Transducer of erbB 2 antibody
    • Transducer of ERBB2 1 antibody
    • Transducer of erbB2 antibody
    • Trob antibody
    • TROB 1 antibody
    • TROB1 antibody
    see all

Anti-TOB antibody 画像

Anti-TOB antibody (ab48826) 使用論文

ab48826 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab48826.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"