The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 32 kDa).
Use at an assay dependent dilution.
Transcription factor TFIIA stabilizes the DNA binding of TATA box-binding protein (TBP). It also plays a very important role in the activation of transcription factor TFIID by the function of the transcription regulator factor. Budding yeast TFIIA is composed of two subunits, Toa1p (286aa) and Toa2p (122aa).